The Author's Response: Bioinformatics Analysis in Downstream Genes of the mTOR Pathway to Predict Recurrence and Progression of Bladder Cancer
نویسنده
چکیده
Due to the high false positive caused by the characteristics of microarray, it is right that gene selection is performed by rational statistical method, based on analysis of variance model and so on. However, in practice, changes in expression of at least 2-fold have been considered significant, although only rarely has any statistical or biological justification been offered for the selection of this threshold.1,2 Of course, the data must be normalized to eliminate systematic variations, which are not related to changes in relative RNA abundance for individual genes.
منابع مشابه
Letter to the Editor: Bioinformatics Analysis in Downstream Genes of the mTOR Pathway to Predict Recurrence and Progression of Bladder Cancer
We read with great interest the recent report by Jin et al.,1 “Identification of Downstream Genes of the mTOR Pathway that Predict Recurrence and Progression in Non-Muscle Invasive High-Grade Urothelial Carcinoma of the Bladder,” which appeared on 7 June 2017 in Journal of Korean Medical Science. The results of the report are very helpful for us, however, from our perspective, the author's meth...
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Microarray analysis was used to investigate the lack of identified mammalian target of rapamycin (mTOR) pathway downstream genes to overcome cross-talk at non-muscle invasive high-grade (HG)-urothelial carcinoma (UC) of the bladder, gene expression patterns, gene ontology, and gene clustering by triple (p70S6K, S6K, and eIF4E) small interfering RNAs (siRNAs) or rapamycin in 5637 and T24 cell li...
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عنوان ژورنال:
دوره 33 شماره
صفحات -
تاریخ انتشار 2018